Applications of cross electrophoresis and Disc electrophoresis
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چکیده
منابع مشابه
Disc Electrophoresis. I. Background and Theory.
Although electrophoresis is one of the most effective methods for the separation of ionic components of a mixture, the resolving power of different electrophoretic methods is quite variable. To separate two component ions, it is necessary to permit migration to continue until one of the kinds of ions has traveled at least one thickness of the volumes that it initially occupied (the starting zon...
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The disc electrophoresis of lipoproteins of unfractionated rat plasma is described. The plasma was prestained with Sudan black B and electrophoretically separated at polyacrylamide gel concentrations of 7.5, 5, and 3.75%. At least four lipoprotein components were observed, and an additional 2-3 components in the main gel and 2-5 components in the spacer gel possibly were present. Densitometry o...
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Column Zorbax silica, 25 cm 4.6 mm internal diameter (7 m) S5W uncoated silica, 25 cm 4.6 mm internal diameter (5 m) Eluent Hexane}ethyl acetate, 95 : 5 CO2 modified with small amounts of methanol Flow rate 1.6 mL min 1 2 mL min 1 for 4 min, then gradient of 2 up to 5 mL min 1 thereafter held constant Programme Isocratic P"100 atm, T"403C; modifier, 1.5%min 1 from 10% to 30% thereafter held con...
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Positive or negative electrical charges are frequently associated with biomolecules. When placed in an electric field, charged biomolecules move towards the electrode of opposite charge due to the phenomenon of electrostatic attraction. Electrophoresis is the separation of charged molecules in an applied electric field. The relative mobility of individual molecules depends on several factors. T...
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After having grown through the stages of technique development and instrumentation availability, capillary electrophoresis has reached the stage of applications. This review attempts to show the potential of this technique for biomedical analysis. Rather than going into a detailed description of the technical details of the separation conditions suitable for the separation of a particular categ...
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 1970
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.15.103